HPTLC Method Development and Validation for Simultaneous Estimation of Berberine, Ellagic Acid and Ferulic Acid in <i>Amrtadi Churna</i>
نویسندگان
چکیده
The objective of the current study was to develop a new simple and precise High Performance Thin Layer Chromatography (HPTLC) method for standardization three biomarkers i.e., berberine, ellagic acid ferulic in Amrtadi churna. churna is mainly used hyperacidity contains dried plant parts Gokshur, Amla Guduchi. developed validated using precoated silica gel at 60 F254 as stationary phase toluene:ethyl acetate:formic acid:methanol (6:6:1.6:0.4, v/v/v/v) mobile phase. detection quantification were performed 319 nm Rf value obtained 0.35±2 0.5±2 0.74±2 acid. per ICH guidelines terms linearity, precision, specificity, accuracy robustness.
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ژورنال
عنوان ژورنال: Journal of Natural Remedies
سال: 2022
ISSN: ['0972-5547', '2320-3358']
DOI: https://doi.org/10.18311/jnr/2022/31350